Department of Histochemistry and Electron microscopy

Head of Department:

Slezák Ján, profesor, MUDr., DrSc.

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Senior Scientists:

Tribulová Narcisa, RNDr., DrSc.

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Okruhlicová Ľudmila, RNDr., CSc.

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Research Assistents:

Bačová Barbara, RNDr., PhD.

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Beňová Tamara, RNDr., PhD.

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Frimmel Karel, Ing., PhD.

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Radošinská Jana, MUDr., PhD.

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PhD Students:

Viczenczová Csilla, Mgr.

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Križák Jakub, Mgr.

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Diploma Students:


Kavecká Marta, Bc., Prírodovedecká fakulta UK, Bratislava


Poľačková Anna, Bc., Prírodovedecká fakulta UK, Bratislava


 

AREAS OF RESEARCH:

  • The study of relation of structure and function of the heart and vessels during physiological and pathophysiological conditions, imitating clinical situations.
  • The study of molecular and subcellular mechanisms in pathogenesis of the myocardial arrhythmias, prevention of cardiovascular injury.
  • The study of gap junction intercellular channels which coordinate propagation of electrical and metabolic signals between adjacent cells.
  • The study of vascular gap junctions during structural remodeling and atherosclerotic processes.
  • The protection of heart muscle and blood vessels against injury via modulation of gap junction connexin channels.
  • The study of heart and vessel protection against ionizing radiation.


EXPERIMENTAL MODELS OF CARDIOVASCULAR DISEASES:

Chronic and acute: hypertension, hypertriglyceridemia, altered thyroid status, aging, hypoxia, ischemia, diabetes mellitus, inflammation, protection of heart and vessels against ionising radiation.


TECHNIQUES:

  • Transmission electron microscopy
  • Basic in situ catalytic enzyme histochemistry
  • Cytochemistry of selected enzymes
  • Immunofluorescence
  • Western blotting of selected proteins
  • Image morphometric analysis


NATIONAL CO-OPERATIONS:

Institute of Experimental Pharmacology and Toxicology, SAS, Bratislava

Vladimír Knezl, RNDr., PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Ružena Sotníková, RNDr., PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Jana Navarová, RNDr., PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Institute of Normal and Pathological Physiology, SAS, Bratislava

Iveta Bernátová, DSc. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Angelika Púszerová, RNDr, PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Medical Faculty, Comenius University, Bratislava

Peter Weismann, RNDr., PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Jessenius Medical Faculty, Comenius University, Martin

Peter Kaplán, Ass.Prof., PhD. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Slovak Medical Faculty in Bratislava

Marko Fülop, M.D., DSc. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Slovak Office of Standards, Metrology and Testing, Bratislava

Ján Ružička, prof., DSc. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Catholic University, Ružomberok

Tadeusz Zasepa, prof. This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Elektrotechnický výskumný a projektový ústav, Nová Dubnica

Jozef Budaj, Ing., CSc., gen riaditeľ This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Národný ústav srdcových a cievnych chorôb, Bratislava

Doc. Eva Goncalvesová, MUDr., CSc.


 

INTERNATIONAL CO-OPERATIONS:

Div. Of Cardiovascular Science, St. Boniface General Hospital, Research Ctr., Manitoba, Canada

Prof. Dhalla N.J., Prof. Singal P.K., Prof. Slezák J.

Physiological Institute, Dept. Functional Morphology, AS CR, Prague, Czech Republic

Soukup T., PhD., Tribulová N., DSc.

Bogomoletz Institute of Physiology, NAS of Ukraine, Kiev, Ukraina

Prof. Moibenko A.A., Tribulová N., DSc.

Protom Protvino, Russia

Prof. Balagin V., Prof. Slezák J.

 

PROJECTS:


International projects:



Principal investigator: Tribulova Narcisa

Co-operation: Institute of Physiology, Academy of Sciences of Czech Republic, Prague



Principal investigator: Tribulova Narcisa

Co-operation: Bogomoletz Institute of Physiology, Kiev, Ukraina


Principal investigator: Tribulova Narcisa

Co-operation: Bogomoletz Institute of Physiology, Kiev, Ukraina


Projects supported by Slovak Research and Development Agency:


Agentúra na podporu výskumu a vývoja (APVV)

Running project

Principal investigator: Bernátová Iveta (Institue of Normal and Pathological Physiology SAS)

Contact person: Okruhlicová Ľudmila

Completed projects

  • APVV-51-059505 Protection of the heart against malignant arrhythmias and heart failure.

Principal investigator: Okruhlicová Ľudmila

Date: 2006-2009


Projects supported by VEGA:


Principal investigator: Slezák Ján

Date: 2011-2013

Principal investigator: Okruhlicová Ľudmila

Date: 2011-2012

Principal investigator: Tribulová Narcisa

Date: 2009-2011


SELECTED PUBLICATIONS:

 

  1. Bačová B, Seč P, Radošinská J, Certík M, Vachulová A, Tribulová N. Lower omega-3 index is a marker of increased propensity of hypertensive rat heart to malignant arrhythmias. Physiol Res. 62(1):S201-208, 2013.
  2. Benova T, Viczenczova C, Radosinska J, Bacova B, Knezl V, Dosenko V, Weismann P, Zeman M, Navarova J,Tribulova N. Melatonin attenuates hypertension-related proarrhythmic myocardial maladaptation of connexin-43 and propensity of the heart to lethal arrhythmias. Can J Physiol Pharmacol. 91(8):633-639, 2013.
  3. Frimmel K, Vlkovicova J, Sotnikova R, Navarova J, Bernatova I, Okruhlicova L.The effect of omega-3 fatty acids on expression of connexin-40 in Wistar rat aorta after lipopolysaccharide administration. J Physiol Pharmacol. 65(1):83-94, 2014.
  4. Viczenczova C, Bacova B, Benova T, Kura B, Yin Ch, Kukreja R, Slezak J, Tribulova N. Irradiation-induced upregulation of myocardial connexin-43 is associated with repression of microRNA-1 in rat heart. Exp Clin Cardiol.18(A):35A-36A, 2013.
  5. Radosinska J., Bacova B., Bernatova I., Navarova J., Zhukovska A., Shysh A., Okruhlicova L., Tribulova N. Myocardial NOS activity and connexin43 expression in untreated and omega-3 fatty acids-treated spontaneously hypertensive and hereditary hypertriglyceridemic rats. Molecular and Cellular Biochemistry, 347, 163-173, 2011
  6. Sotnikova R., Nedelčevova J., Navarova J., Nosalova V., Drabikova K., Szocs K., Krenek P., Kyselova Z., Bezek S., Knezl V., Drimal J., Broskova Z., Kristova V., Okruhlicova L, Bernatova I., Bauer V. Protection of the vascular endothelium in experimental situations. Interdisc Toxicol 4, 1, 20-26, 2011.
  7. Okruhlicova L., Gabauer I., Slezak J. Ultraštruktúrny obraz koronárnych artérií a aorty pacientov s dilatačnou kardiomyopatiou. Cardiology Letters, 3, p.xxx, 2011.
  8. Bacova B., Radosinska J., Knezl V., Kolenova L., Weismann P., Navarova J., Barancik M., Mitasikova M., Tribulova N.Omega-3 fatty acids and atorvastatin suppress ventricular fibrillation inducibility in hypertriglyceridemic rat hearts: implication of intracellular coupling protein, connexin-43. J Physiol Pharmacol 61, 6, 717-723, 2010.
  9. Dhingra S., Sharma A.K., Arora R.C., Slezák J., Singal P.K. IL-10 attenuates TNF-alpha-induced NF kappa B pathway activation and cardiomyocyte apoptosis. Cardiovascular Research, vol. 82, issue 1, p. 59-66, 2009.
  10. Dlugošová K., Weismann P., Bernátová I., Sotníková R., Slezák J., Okruhlicová Ľ. Omega-3 fatty acids and atorvastatin affect connexin 43 expression in the aorta hereditary hypertriglyceridemic rats. In Canadian Journal of Physiology and Pharmacology, vol. 87, no. 12, p. 1074 – 1082, 2009.
  11. Slezák J., Tribulová N., Okruhlicová Ľ., Dhingra R., Bajaj A., Freed D., Singal P. Hibernating myocardium: pathophysiology, diagnosis, and treatment. In Canadian Journal of Physiology and Pharmacology, vol. 87, issue 4, p. 252-265, 2009.
  12. Okruhlicova L., Morwinski R., Schulze W., Bartel S., Weismann P., Tribulova N., Wallukat G. Autoantibodies against G-protein-coupled receptors modulate heart mast cells. Cell Mol Immunol 4, no.2, 127-133, 2007